Cast / Details
| Shia LaBeouf | Sam Witwicky |
| Tyrese Gibson | USAF Tech Sergeant Epps |
| Hugo Weaving | Megatron |
| Josh Duhamel | Captain Lennox |
| Megan Fox | Mikaela Banes |
| Isabel Lucas | Alice |
| Samantha Smith | Sarah Lennox |
| Mark Ryan | Bumblebee (voice) |
| Darius McCrary | Jazz (voice) |
| Kevin Dunn | Ron Witwicky |
| Jess Harnell | Ironhide / Barricade (voice) |
| Robert Foxworth | Ratchet (voice) |
| Rainn Wilson | College Professor |
| John Turturro | Agent Simmons/Voice of Jetfire |
| Charles Adler | Starscream (voice) |
| Peter Cullen | Optimus Prime (voice) |
| Matthew Marsden | Graham |
| Frank Welker | Soundwave (voice) |
| Mike Patton | Mixmaster (voice) |